Diversification of the primary antibody repertoire begins during early follicle development in the rabbit appendix Journal Article

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Authors: Zhai, S. K.; Lanning, D. K.
Article Title: Diversification of the primary antibody repertoire begins during early follicle development in the rabbit appendix
Abstract: Rabbits generate a diversified primary antibody repertoire by somatically mutating, in gut-associated lymphoid tissue (GALT), an initial repertoire that is limited by preferential rearrangement of the 3'-most IGVH gene segment. To determine when repertoire diversification begins in GALT, we performed in situ hybridization on neonatal rabbit appendix sections with an activation-induced cytidine deaminase (AID) riboprobe, because AID is required for the mutational processes that diversify the primary antibody repertoire. We first detected AID mRNA expression around 1 week of age, in the basal region of developing follicles. By PCR-amplifying V-D-J genes from AID mRNA(+) B cells isolated by laser capture microdissection, we found evidence of somatic hypermutation, and one likely instance of somatic gene conversion. Our results suggest that V-(D)-J gene diversification begins during early postnatal appendix development, in B cells stimulated to enter a proliferative program by signals derived from select intestinal commensals.
Journal Title: Molecular immunology
Volume: 54
Issue: 2
ISSN: 1872-9142; 0161-5890
Publisher: Unknown  
Journal Place: England
Date Published: 2013
Start Page: 140
End Page: 147
Language: eng
DOI/URL:

10.1016/j.molimm.2012.11.010

10.1016/j.molimm.2012.11.010
Notes: ID: 13105; CI: Copyright (c) 2012; JID: 7905289; EC 3.5.4.5 (Cytidine Deaminase); 2011/10/20 [received]; 2012/11/12 [revised]; 2012/11/12 [accepted]; 2012/12/25 [aheadofprint]; ppublish