Pulmonary inflammation after ethanol exposure and burn injury is attenuated in the absence of IL-6 Journal Article

Authors: Chen, M. M.; Bird, M. D.; Zahs, A.; Deburghgraeve, C.; Posnik, B.; Davis, C. S.; Kovacs, E. J.
Article Title: Pulmonary inflammation after ethanol exposure and burn injury is attenuated in the absence of IL-6
Abstract: Alcohol consumption leads to an exaggerated inflammatory response after burn injury. Elevated levels of interleukin-6 (IL-6) in patients are associated with increased morbidity and mortality after injury, and high systemic and pulmonary levels of IL-6 have been observed after the combined insult of ethanol exposure and burn injury. To further investigate the role of IL-6 in the pulmonary inflammatory response, we examined leukocyte infiltration and cytokine and chemokine production in the lungs of wild-type and IL-6 knockout mice given vehicle or ethanol (1.11 g/kg) and subjected to a sham or 15% total body surface area burn injury. Levels of neutrophil infiltration and neutrophil chemoattractants were increased to a similar extent in wild-type and IL-6 knockout mice 24 h after burn injury. When ethanol exposure preceded the burn injury, however, a further increase of these inflammatory markers was seen only in the wild-type mice. Additionally, signal transducer and activator of transcription-3 (STAT3) phosphorylation did not increase in response to ethanol exposure in the IL-6 knockout mice, in contrast to their wild-type counterparts. Visual and imaging analysis of alveolar wall thickness supported these findings and similar results were obtained by blocking IL-6 with antibody. Taken together, our data suggest a causal relationship between IL-6 and the excessive pulmonary inflammation observed after the combined insult of ethanol and burn injury.
Journal Title: Alcohol (Fayetteville, N.Y.)
Volume: 47
Issue: 3
ISSN: 1873-6823; 0741-8329
Publisher: Unknown  
Journal Place: United States
Date Published: 2013
Start Page: 223
End Page: 229
Language: eng
Notes: ID: 12994; CI: Copyright (c) 2013; GR: F31 AA019913/AA/NIAAA NIH HHS/United States; GR: F32AA018068/AA/NIAAA NIH HHS/United States; GR: R01 AA012034/AA/NIAAA NIH HHS/United States; GR: R01AA012034/AA/NIAAA NIH HHS/United States; GR: T32 AA013527/AA/NIAAA NIH HHS/United States; GR: T32AA013527/AA/NIAAA NIH HHS/United States; JID: 8502311; NIHMS449170; OID: NLM: NIHMS449170 [Available on 05/01/14]; OID: NLM: PMC3617054 [Available on 05/01/14]; PMCR: 2014/05/01 00:00; 2012/07/03 [received]; 2013/01/04 [revised]; 2013/01/23 [accepted]; 2013/02/23 [aheadofprint]; ppublish