A potential role for B cells in suppressed immune responses in cord blood transplant recipients Journal Article

Authors: Beaudette-Zlatanova, B. C.; Le, P. T.; Knight, K. L.; Zhang, S; Zakrzewski, S.; Parthasarathy, M.; Stiff, P. J.
Article Title: A potential role for B cells in suppressed immune responses in cord blood transplant recipients
Abstract: We evaluated immune reconstitution in 58 adults who received hematopoietic SCTs from allogeneic siblings (allosib), matched unrelated donors (MUD) or cord blood (CB) at 90-day intervals for 1 year post transplant. CB recipients had a higher incidence of infections in the first 100 days compared with allosib and MUD recipients. The number of circulating T cells was lower in CB recipients compared with MUD recipients at 90 days and compared with allosib recipients at 180 days. Spectratype analysis of the TCR Vbeta complementarity determining region 3 (CDR3) of patient lymphocytes revealed that the TCR repertoire remained poorly diversified even at 360 days in nearly all patients. In contrast, the number of circulating B cells was significantly elevated in CB recipients compared with allosib recipients throughout the first year post transplant and compared with MUD recipients at 9-12 months. Spectratype analysis of the B-cell receptor V(H) CDR3 showed that the B-cell repertoire was diversified in most patients by 90 days. CD5(pos) B cells from assayed CB recipients expressed intracellular IL-10 early post transplant. Our data suggest that B cells, in addition to T cells, may have a role in impaired immune responses in CB transplant patients.
Journal Title: Bone marrow transplantation
Volume: 48
Issue: 1
ISSN: 1476-5365; 0268-3369
Publisher: Unknown  
Journal Place: England
Date Published: 2013
Start Page: 85
End Page: 93
Language: eng
Notes: ID: 13182; GR: AG023809/AG/NIA NIH HHS/United States; GR: AI068390/AI/NIAID NIH HHS/United States; GR: F32HL096278/HL/NHLBI NIH HHS/United States; GR: R01 AI068390/AI/NIAID NIH HHS/United States; JID: 8702459; 2012/06/25 [aheadofprint]; ppublish