Distribution and function of polycystin-2 in mouse retinal ganglion cells Journal Article

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Authors: Kaja, S.; Mafe, O. A.; Parikh, R. A.; Kandula, P.; Reddy, C. A.; Gregg, E. V.; Xin, H.; Mitchell, P; Grillo, M. A.; Koulen, P.
Article Title: Distribution and function of polycystin-2 in mouse retinal ganglion cells
Abstract: The polycystin family of transient receptor potential (TRP) channels form Ca(2+) regulated cation channels with distinct subcellullar localizations and functions. As part of heteromultimeric channels and multi-protein complexes, polycystins control intracellular Ca(2+) signals and more generally the translation of extracellular signals and stimuli to intracellular responses. Polycystin-2 channels have been cloned from retina, but their distribution and function in retinal ganglion cells (RGCs) have not yet been established. In the present study, we determined cellular and subcellular localization as well as functional properties of polycystin-2 channels in RGCs. Polycystin-2 expression and distribution in RGCs was assessed by immunohistochemistry on vertical cryostat section of mouse retina as well as primary cultured mouse RGCs, using fluorescence microscopy. Biophysical and pharmacological properties of polycystin-2 channels isolated from primary cultured RGCs were determined using planar lipid bilayer electrophysiology. We detected polycystin-2 immunoreactivity both in the ganglion cell layer as well as in primary cultured RGCs. Subcellular analysis revealed strong cytosolic localization pattern of polycystin-2. Polycystin-2 channel current was Ca(2+) activated, had a maximum slope conductance of 114 pS, and could be blocked in a dose-dependent manner by increasing concentrations of Mg(2+). The cytosolic localization of polycystin-2 in RGCs is in accordance with its function as intracellular Ca(2+) release channel. We conclude that polycystin-2 forms functional channels in RGCs, of which biophysical and pharmacological properties are similar to polycystin-2 channels reported for other tissues and organisms. Our data suggest a potential role for polycystin-2 in RGC Ca(2+) signaling.
Journal Title: Neuroscience
Volume: 202
ISSN: 1873-7544; 0306-4522
Publisher: IBRO. Published by Elsevier Ltd  
Journal Place: United States
Date Published: 2012
Start Page: 99
End Page: 107
Language: ENG
DOI/URL:

10.1016/j.neuroscience.2011.11.047
Notes: LR: 20161019; CI: Copyright (c) 2011; GR: P01 AG022550/AG/NIA NIH HHS/United States; GR: S10 RR022570-01/RR/NCRR NIH HHS/United States; GR: P01 AG027956/AG/NIA NIH HHS/United States; GR: P01 AG010485-18/AG/NIA NIH HHS/United States; GR: AG010485/AG/NIA NIH HHS/United States; GR: R01 EY014227/EY/NEI NIH HHS/United States; GR: AG022550/AG/NIA NIH HHS/United States; GR: P01 AG010485/AG/NIA NIH HHS/United States; GR: P01 AG022550-09/AG/NIA NIH HHS/United States; GR: EY014227/EY/NEI NIH HHS/United States; GR: S10 RR027093-01/RR/NCRR NIH HHS/United States; GR: P01 AG027956-04/AG/NIA NIH HHS/United States; GR: RR022570/RR/NCRR NIH HHS/United States; GR: AG027956/AG/NIA NIH HHS/United States; GR: R01 EY014227-04/EY/NEI NIH HHS/United States; GR: S10 RR027093/RR/NCRR NIH HHS/United States; GR: S10 RR022570/RR/NCRR NIH HHS/United States; GR: RR027093/RR/NCRR NIH HHS/United States; JID: 7605074; 0 (Ion Channels); 0 (Lipid Bilayers); 0 (TRPP Cation Channels); 0 (polycystic kidney disease 2 protein); I38ZP9992A (Magnesium); NIHMS342441; OID: NLM: NIHMS342441; OID: NLM: PMC3268838; 2011/08/28 [received]; 2011/11/22 [revised]; 2011/11/25 [accepted]; ppublish