Alveolar macrophage inflammatory mediator expression is elevated in the setting of alcohol use disorders Journal Article

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Authors: O'Halloran, E. B.; Curtis, B. J.; Afshar, M; Chen, M. M.; Kovacs, E. J.; Burnham, E. L.
Article Title: Alveolar macrophage inflammatory mediator expression is elevated in the setting of alcohol use disorders
Abstract: Alcohol use disorders (AUDs) are associated with increased susceptibility to pulmonary diseases, including bacterial pneumonia and acute respiratory distress syndrome (ARDS). Alveolar macrophages (AMs) play a vital role in the clearance of pathogens and regulation of inflammation, but these functions may be impaired in the setting of alcohol exposure. We examined the effect of AUDs on profiles of cytokines, chemokines, and growth factors in human AMs isolated from bronchoalveolar lavage (BAL) samples from 19 AUD subjects and 20 age-, sex-, and smoking-matched control subjects. By multiplex bead array, the lysates of AMs from subjects with AUDs had significant elevation in the cytokine tumor necrosis factor alpha (TNF-alpha), as well as chemokine (C-X-C motif) ligand 8 (CXCL8), CXCL10, and chemokine (C-C motif) ligand 5 (CCL5) (p 0.05). Additionally, a 1.8-fold increase in IL-1beta, 2.0-fold increase in IL-6, 2.3-fold increase in interferon gamma (IFN-gamma), 1.4-fold increase in CCL3, and a 2.3-fold increase in CCL4 was observed in the AUD group as compared to the control group. We also observed compensatory increases in the anti-inflammatory cytokine IL-1RA (p 0.05). AUD subjects had 5-fold higher levels of CXCL11 mRNA expression (p 0.05) and a 2.4-fold increase in IL-6 mRNA expression by RT-PCR as well. In these investigations, alcohol use disorders were associated with functional changes in human AMs, suggesting that chronic alcohol exposure portends a chronically pro-inflammatory profile in these cells.
Journal Title: Alcohol (Fayetteville, N.Y.)
Volume: 50
ISSN: 1873-6823; 0741-8329
Publisher: Unknown  
Journal Place: United States
Date Published: 2016
Start Page: 43
End Page: 50
Language: eng
DOI/URL:

10.1016/j.alcohol.2015.11.003
Notes: LR: 20160224; CI: Copyright (c) 2016; GR: R01 AA012034/AA/NIAAA NIH HHS/United States; GR: R01 GM115257/GM/NIGMS NIH HHS/United States; GR: R24 AA019661/AA/NIAAA NIH HHS/United States; GR: T32 AA013527/AA/NIAAA NIH HHS/United States; GR: UL1 RR025780/RR/NCRR NIH HHS/United States; GR: UL1 TR001082/TR/NCATS NIH HHS/United States; JID: 8502311; NIHMS750786; OID: NLM: NIHMS750786 [Available on 02/01/17]; OID: NLM: PMC4753084 [Available on 02/01/17]; OTO: NOTNLM; PMCR: 2017/02/01 00:00; 2015/04/13 [received]; 2015/11/02 [revised]; 2015/11/06 [accepted]; 2015/11/24 [aheadofprint]; ppublish