Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer Journal Article

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Authors: Yan, J.; Thomson, J. K.; Zhao, W; Fast, V. G.; Ye, T.; Ai, X
Article Title: Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer
Abstract: Optical mapping has proven to be a valuable technique to detect cardiac electrical activity on both intact ex vivo hearts and in cultured myocyte monolayers. HL-1 cells have been widely used as a 2-Dimensional cellular model for studying diverse aspects of cardiac physiology. However, it has been a great challenge to optically map calcium (Ca) transients and action potentials simultaneously from the same field of view in a cultured HL-1 atrial cell monolayer. This is because special handling and care is required to prepare healthy cells that can be electrically captured and optically mapped. Therefore, we have developed an optimal working protocol for dual channel optical mapping. In this manuscript, we have described in detail how to perform the dual channel optical mapping experiment. This protocol is a useful tool to enhance the understanding of action potential propagation and Ca kinetics in arrhythmia development.
Journal Title: Journal of visualized experiments : JoVE
Volume: (97). doi
Issue: 97
ISSN: 1940-087X; 1940-087X
Publisher: Unknown  
Journal Place: United States
Date Published: 2015
Start Page: 10.3791/52542
Language: eng
DOI/URL:

10.3791/52542
Notes: LR: 20150415; GR: R01 HL113640/HL/NHLBI NIH HHS/United States; JID: 101313252; epublish
LUC Authors
  1. Xun Ai
    10 Ai
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