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Increased expression of secreted frizzled-related protein-1 and microtubule-associated protein light chain 3 in keratoconus Journal Article
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| Authors: | Iqbal, O; Fisher, G.; Vira, S.; Syed, D.; Sadeghi, N; Freeman, D.; Campbell, E; Sugar, J.; Feder, R.; Fareed, J; Bouchard, C |
| Article Title: | Increased expression of secreted frizzled-related protein-1 and microtubule-associated protein light chain 3 in keratoconus |
| Abstract: | PURPOSE: To study the expression of secreted frizzled-related protein-1 (SFRP-1) and microtubule-associated protein light chain 3 (LC3), an autophagy marker, in keratoconus. METHODS: Under an institutional review board-approved protocol, de-identified and/or surgically discarded normal donor (n = 10) and keratoconus corneas (n = 10) were obtained. The corneal samples were fixed in formalin and embedded in paraffin. Immunohistochemical staining using SFRP-1 and LC3 antibodies was performed. RESULTS: The majority of expression of SFRP-1 was seen in the epithelium; however, in 3 tissues that showed high expression, staining was also present in the stroma and endothelium. Like SFRP-1, the LC3 expression in keratoconus tissues occurred at 3 different levels: low, medium, and high. Collectively these data suggest that there are differences in the expression levels of SFRP-1 and LC3 in keratoconus tissue compared with the normal tissue. Low expressivity of SFRP-1 seemed to correspond to low expressivity of LC3, whereas medium to high expressivity of SFRP-1 corresponded to medium to high expressivity of LC3. CONCLUSIONS: Increased expression of SFRP-1 and LC3 was observed in keratoconus corneas. Keratocyte autophagy seen with keratoconus may play a role in the pathogenesis of keratoconus. |
| Journal Title: | Cornea |
| Volume: | 32 |
| Issue: | 5 |
| ISSN: | 1536-4798; 0277-3740 |
| Publisher: | Unknown |
| Journal Place: | United States |
| Date Published: | 2013 |
| Start Page: | 702 |
| End Page: | 707 |
| Language: | eng |
| DOI/URL: | |
| Notes: | ID: 13002; JID: 8216186; ppublish |
