Consumption of high fat diet acutely prior to ischemia/reperfusion results in cardioprotection through NF-kappaB dependent regulation of autophagic pathways Journal Article


Authors: Haar, L; Ren, X; Liu, Y; Koch, S. E.; Goines, J.; Tranter, M.; Engevik, M. A.; Nieman, M. L.; Rubinstein, J.; Jones, W. K.
Article Title: Consumption of high fat diet acutely prior to ischemia/reperfusion results in cardioprotection through NF-kappaB dependent regulation of autophagic pathways
Abstract: Previous studies have demonstrated improvement of cardiac function occurs with acute consumption high fat diet (HFD) after myocardial infarction (MI). However, no data exists addressing the effects of acute HFD upon the extent of injury after MI. This study investigates the hypothesis that short term HFD, prior to infarction, protects the heart against I/R injury through NF-kappaB-dependent regulation of cell death pathways in the heart. Data shows that acute HFD initiates cardioprotection against MI (>50% reduction in infarct size normalized to risk region) after 24h to 2 weeks of HFD, but protection is completely absent after 6 weeks of HFD, when mice are reported to develop pathophysiology related to the diet. Furthermore, cardioprotection after 24h of HFD persists after an additional 24h of normal chow feeding, and was found to be dependent upon NF-kappaB activation in cardiomyocytes. This study also indicates that short term HFD activates autophagic processes (Beclin-1, LC-3) pre-ischemia, as seen in other protective stimuli. Increases in Beclin-1 and LC-3 were found to be NF-kappaB-dependent, and administration of chloroquine, an inhibitor of autophagy, abrogated cardioprotection. Our results support that acute high fat feeding mediates cardioprotection against I/R injury associated with a NF-kappaB-dependent increase in autophagy and reduced apoptosis, as has been found for ischemic preconditioning.
Journal Title: American journal of physiology.Heart and circulatory physiology
Volume: 307
Issue: 12
ISSN: 1522-1539; 0363-6135
Publisher: American Journal of Physiology - Heart and Circulatory Physiology  
Date Published: 2014
Start Page: H1705
End Page: H1713
Language: ENG
DOI/URL:
Notes: CI: Copyright (c) 2014; JID: 100901228; OTO: NOTNLM; aheadofprint
LUC Authors
  1. W. Keith Jones
    18 Jones
  2. Xiaoping Ren
    8 Ren
  3. Yuk-Ming Liu
    47 Liu
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