Monitoring of inflammation using novel biosensor mouse model reveals tissue- and sex-specific responses to western diet. Journal Article

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Authors: Talley, S; Bonomo, R; Gavini, C; Hatahet, J; Gornick, E; Cook, T; Chun, B; Kekenes-Huskey, P; Aubert, G; Campbell, E; Mansuy-Aubert, V
Article Title: Monitoring of inflammation using novel biosensor mouse model reveals tissue- and sex-specific responses to western diet.
Abstract: Obesity is an epidemic, and it is characterized by a state of low-grade systemic inflammation A key component of inflammation is the activation of inflammasomes, multiprotein complexes that form in response to danger signals and that lead to activation of caspase-1. Previous studies have found that a Westernized diet induces activation of inflammasomes and production of inflammatory cytokines. Gut microbiota metabolites, including the short-chain fatty acid butyrate, have received increased attention as underlying some obesogenic features, but the mechanisms of action by which butyrate influences inflammation in obesity remain unclear. We engineered a caspase-1 reporter mouse model to measure spatiotemporal dynamics of inflammation in obese mice. Concurrent with increased capsase-1 activation in vivo, we detected stronger biosensor signal in white adipose and heart tissues of obese mice ex vivo and observed that a short-term butyrate treatment affected some but not all the inflammatory responses induced by Western diet. Through characterization of inflammatory responses and computational analyses, we identified tissue- and sex-specific caspase-1 activation patterns and inflammatory phenotypes in obese mice, offering new mechanistic insights underlying the dynamics of inflammation.
Journal Title: Disease models mechanisms
Publisher: Unknown  
Date Published: 2022