Quantification of Lactate Dehydrogenase for Cell Viability Testing Using Cell Lines and Primary Cultured Astrocytes Journal Article

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Authors: Kaja, S.; Payne, A. J.; Naumchuk, Y.; Koulen, P.
Article Title: Quantification of Lactate Dehydrogenase for Cell Viability Testing Using Cell Lines and Primary Cultured Astrocytes
Abstract: Drug discovery heavily relies on cell viability studies to assess the potential toxicity of drug candidates. L-Lactate dehydrogenase (LDH) is a cytoplasmic enzyme that catalyzes the concomitant interconversions of pyruvate to L-lactate and NADH to NAD(+) during glycolysis, and the reverse reactions during the Cori cycle. In response to cellular damage, induced by endogenous cellular mechanisms or as a result of exogenously applied insults, LDH is released from the cytoplasm into the extracellular environment. Its stability in cell culture medium makes it a well-suited correlate for the presence of damage and toxicity in tissues and cells. We herein present protocols for a reproducible and validated LDH assay optimized for several cell types. In contrast to commercially available LDH assays, often associated with proprietary formulations and high cost, our protocols provide ample opportunities for experiment-specific optimization with low variability and cost. © 2017 by John Wiley & Sons, Inc.
Journal Title: Current protocols in toxicology
Volume: 72
ISSN: 1934-9262
Publisher: John Wiley & Sons, Inc  
Date Published: 2017
Start Page: 2.26.1
End Page: 2.26.10
Language: eng
DOI/URL:

10.1002/cptx.21
Notes: LR: 20181113; CI: Copyright © 2017; GR: P01 AG022550/AG/NIA NIH HHS/United States; GR: P01 AG027956/AG/NIA NIH HHS/United States; GR: R01 EY022774/EY/NEI NIH HHS/United States; GR: S10 RR027093/RR/NCRR NIH HHS/United States; JID: 9816330; 0 (Culture Media); 0 (Neuroprotective Agents); EC 1.1.1.27 (L-Lactate Dehydrogenase); NIHMS871020; OTO: NOTNLM; 2017/05/03 06:00 [entrez]; 2017/05/04 06:00 [pubmed]; 2018/03/09 06:00 [medline]; epublish