Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines. Journal Article

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Authors: O'Brien, A; Mettelman, RC; Volk, A; André, NM; Whittaker, GR; Baker, SC
Article Title: Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines.
Abstract: Investigating type I feline coronaviruses (FCoVs) in tissue culture is critical for understanding the basic virology, pathogenesis, and virus-host interactome of these important veterinary pathogens. This has been a perennial challenge as type I FCoV strains do not easily adapt to cell culture. Here we characterize replication kinetics and plaque formation of a model type I strain FIPV Black in Fcwf-4 cells established at Cornell University (Fcwf-4 CU). We determined that maximum virus titers (>10 pfu/mL) were recoverable from infected Fcwf-4 CU cell-free supernatant at 20?h post-infection. Type I FIPV Black and both biotypes of type II FCoV formed uniform and enumerable plaques on Fcwf-4 CU cells. Therefore, these cells were employable in a standardized plaque assay. Finally, we determined that the Fcwf-4 CU cells were morphologically distinct from feline bone marrow-derived macrophages and were less sensitive to exogenous type I interferon than were Fcwf-4 cells purchased from ATCC.
Journal Title: Virology
ISSN: 1096-0341; 0042-6822
Publisher: Elsevier Inc  
Date Published: 2018