Authors: | Deng, X; Baker, S. C. |
Article Title: | An "old" protein with a new story: Coronavirus endoribonuclease is important for evading host antiviral defenses |
Abstract: | Here we review the evolving story of the coronavirus endoribonuclease (EndoU). Coronavirus EndoU is encoded within the sequence of nonstructural protein (nsp) 15, which was initially identified as a component of the viral replication complex. Biochemical and structural studies revealed the enzymatic nature of nsp15/EndoU, which was postulated to be essential for the unique replication cycle of viruses in the order Nidovirales. However, the role of nsp15 in coronavirus replication was enigmatic as EndoU-deficient coronaviruses were viable and replicated to near wild-type virus levels in fibroblast cells. A breakthrough in our understanding of the role of EndoU was revealed in recent studies, which showed that EndoU mediates the evasion of viral double-stranded RNA recognition by host sensors in macrophages. This new discovery of nsp15/EndoU function leads to new opportunities for investigating how a viral EndoU contributes to pathogenesis and exploiting this enzyme for therapeutics and vaccine design against pathogenic coronaviruses. |
Journal Title: | Virology |
Volume: | 517 |
ISSN: | 1096-0341; 0042-6822 |
Publisher: | Elsevier Inc |
Journal Place: | United States |
Date Published: | 2018 |
Start Page: | 157 |
End Page: | 163 |
Language: | eng |
DOI/URL: |
S0042-6822(17)30437-3 |
Notes: | LR: 20180420; CI: Copyright (c) 2018; GR: R01 AI085089/AI/NIAID NIH HHS/United States; JID: 0110674; 0 (Viral Nonstructural Proteins); EC 3.1.- (Endoribonucleases); NIHMS932488; OTO: NOTNLM; PMCR: 2019/04/01 00:00; 2017/10/04 00:00 [received]; 2017/12/21 00:00 [revised]; 2017/12/22 00:00 [accepted]; 2019/04/01 00:00 [pmc-release]; 2018/01/09 06:00 [pubmed]; 2018/04/21 06:00 [medline]; 2018/01/09 06:00 [entrez]; ppublish |