Cell intrinsic characteristics of human cord blood naive CD4T cells Journal Article

Authors: Jacks, R. D.; Keller, T. J.; Nelson, A; Nishimura, M. I.; White, P; Iwashima, M
Article Title: Cell intrinsic characteristics of human cord blood naive CD4T cells
Abstract: It has been generally considered that the perinatal immune system is less inflammatory compared to the adult system and type 2 responses predominate perinatal immune responses against antigens. Indeed, previous studies in mice showed that there are cell-intrinsic differences between neonatal and adult CD4T cells. However, studies on human cord blood and infant blood demonstrated that human perinatal T cells do not produce elevated levels of Th2 cytokines with the exception of IL-13. These data raise the question if human T cells in the perinatal blood fundamentally differ from adult T cells. To decipher differences between human perinatal and adult T cells, we performed a focused comparative analysis on purified naive CD4T cells from umbilical cord blood (UCB) and adult peripheral blood. Our data demonstrate naive CD4T cells from UCB differ from adult naive CD4T cells in surface expression of CD26, dipeptidyl peptidase-4. While only a fraction of effector/memory T cells from adult blood express CD26, practically all T cells from UCB express high levels of CD26. We also determined that Th1/Th2 polarizing conditions induce UCB CD4T cells to produce higher levels of IFN-gamma and IL-5 compared to adult CD4T cells, respectively. These data demonstrate intrinsic differences between UCB and adult naive CD4T cells and suggest that human perinatal immune responses involve more complex mechanisms than the previously thought Th2-dominant responses.
Journal Title: Immunology letters
Volume: 193
ISSN: 1879-0542; 0165-2478
Publisher: European Federation of Immunological Societies. Published by Elsevier B.V  
Journal Place: Netherlands
Date Published: 2018
Start Page: 51
End Page: 57
Language: eng
Notes: LR: 20180104; CI: Copyright (c) 2017; GR: P01 CA154778/CA/NCI NIH HHS/United States; GR: R01 AI100129/AI/NIAID NIH HHS/United States; JID: 7910006; NIHMS925501; OTO: NOTNLM; PMCR: 2019/01/01 00:00; 2017/08/28 00:00 [received]; 2017/10/28 00:00 [revised]; 2017/11/22 00:00 [accepted]; 2019/01/01 00:00 [pmc-release]; 2017/11/29 06:00 [pubmed]; 2017/11/29 06:00 [medline]; 2017/11/29 06:00 [entrez]; ppublish