An improved protocol for harvesting Bacillus subtilis colony biofilms Journal Article

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Authors: Fuchs, F. M.; Driks, A; Setlow, P.; Moeller, R.
Article Title: An improved protocol for harvesting Bacillus subtilis colony biofilms
Abstract: Bacterial biofilms cause severe problems in medicine and industry due to the high resistance to disinfectants and environmental stress of organisms within biofilms. Addressing challenges caused by biofilms requires full understanding of the underlying mechanisms for bacterial resistance and survival in biofilms. However, such work is hampered by a relative lack of systems for biofilm cultivation that are practical and reproducible. To address this problem, we developed a readily applicable method to culture Bacillus subtilis biofilms on a membrane filter. The method results in biofilms with highly reproducible characteristics, and which can be readily analyzed by a variety of methods with little further manipulation. This biofilm preparation method simplifies routine generation of B. subtilis biofilms for molecular and cellular analysis, and could be applicable to other microbial systems.
Journal Title: Journal of microbiological methods
Volume: 134
ISSN: 1872-8359; 0167-7012
Publisher: Elsevier Inc  
Journal Place: Netherlands
Date Published: 2017
Start Page: 7
End Page: 13
Language: eng
DOI/URL:

S0167-7012(17)30002-7
Notes: LR: 20170522; CI: Copyright (c) 2017; JID: 8306883; OTO: NOTNLM; 2016/11/08 [received]; 2017/01/03 [revised]; 2017/01/03 [accepted]; ppublish