FAK deletion accelerates liver regeneration after two-thirds partial hepatectomy Journal Article

Authors: Shang, N; Arteaga, M; Chitsike, L; Wang, F; Viswakarma, N.; Breslin, P; Qiu, W
Article Title: FAK deletion accelerates liver regeneration after two-thirds partial hepatectomy
Abstract: Understanding the molecular mechanisms of liver regeneration is essential to improve the survival rate of patients after surgical resection of large amounts of liver tissue. Focal adhesion kinase (FAK) regulates different cellular functions, including cell survival, proliferation and cell migration. The role of FAK in liver regeneration remains unknown. In this study, we found that Fak is activated and induced during liver regeneration after two-thirds partial hepatectomy (PHx). We used mice with liver-specific deletion of Fak and investigated the role of Fak in liver regeneration in 2/3 PHx model (removal of 2/3 of the liver). We found that specific deletion of Fak accelerates liver regeneration. Fak deletion enhances hepatocyte proliferation prior to day 3 post-PHx but attenuates hepatocyte proliferation 3 days after PHx. Moreover, we demonstrated that the deletion of Fak in liver transiently increases EGFR activation by regulating the TNFalpha/HB-EGF axis during liver regeneration. Furthermore, we found more apoptosis in Fak-deficient mouse livers compared to WT mouse livers after PHx. CONCLUSION: Our data suggest that Fak is involved in the process of liver regeneration, and inhibition of FAK may be a promising strategy to accelerate liver regeneration in recipients after liver transplantation.
Journal Title: Scientific reports
Volume: 6
ISSN: 2045-2322; 2045-2322
Publisher: Unknown  
Journal Place: England
Date Published: 2016
Start Page: 34316
Language: ENG
Notes: LR: 20161019; GR: R01 CA197128/CA/NCI NIH HHS/United States; GR: R03 CA184652/CA/NCI NIH HHS/United States; GR: R03 CA195183/CA/NCI NIH HHS/United States; JID: 101563288; 2016/05/10 [received]; 2016/09/12 [accepted]; epublish