PLK-1 Silencing in Bladder Cancer by siRNA Delivered With Exosomes Journal Article

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Authors: Greco, K. A.; Franzen, C. A.; Foreman, K. E.; Flanigan, R.C.; Kuo, P. C.; Gupta, G. N.
Article Title: PLK-1 Silencing in Bladder Cancer by siRNA Delivered With Exosomes
Abstract: OBJECTIVE: To use exosomes as a vector to deliver small interfering ribonucleic acid (siRNA) to silence the polo-like kinase 1 (PLK-1) gene in bladder cancer cells. MATERIALS AND METHODS: Exosomes were isolated from both human embryonic kidney 293 (HEK293) cell and mesenchymal stem cell (MSC) conditioned media. Fluorescently labeled exosomes were co-cultured with bladder cancer and normal epithelial cells and uptake was quantified by image cytometry. PLK-1 siRNA and negative control siRNA were loaded into HEK293 and MSC exosomes using electroporation. An invasive bladder cancer cell line (UMUC3) was co-cultured with the electroporated exosomes. Quantitative reverse transcriptase polymerase chain reaction was performed. Protein analysis was performed by Western blot. Annexin V staining and MTT assays were used to investigate effects on apoptosis and viability. RESULTS: Bladder cancer cell lines internalize an increased percentage of HEK293 exosomes when compared to normal bladder epithelial cells. Treatment of UMUC3 cells with exosomes electroporated with PLK-1 siRNA achieved successful knockdown of PLK-1 mRNA and protein when compared to cells treated with negative control exosomes. CONCLUSION: HEK293 and MSC exosomes were effectively used as a delivery vector to transport PLK-1 siRNA to bladder cancer cells in vitro, resulting in selective gene silencing of PLK-1. The use of exosomes as a delivery vector for potential intravesical therapy is attractive.
Journal Title: Urology
Volume: 91
ISSN: 1527-9995; 0090-4295
Publisher: Elsevier Inc  
Journal Place: United States
Date Published: 2016
Start Page: 241.e1
End Page: 241.e7
Language: eng
DOI/URL:

10.1016/j.urology.2016.01.028
Notes: CI: Copyright (c) 2016; JID: 0366151; 2015/07/17 [received]; 2015/12/23 [revised]; 2016/01/22 [accepted]; 2016/02/12 [aheadofprint]; ppublish