Release kinetics of circulating cardiac myosin binding protein-C following cardiac injury Journal Article

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Authors: Kuster, D. W.; Cardenas-Ospina, A.; Miller, L; Liebetrau, C.; Troidl, C.; Nef, H. M.; Mollmann, H.; Hamm, C. W.; Pieper, K. S.; Mahaffey, K. W.; Kleiman, N. S.; Stuyvers, B. D.; Marian, A. J.; Sadayappan, S
Article Title: Release kinetics of circulating cardiac myosin binding protein-C following cardiac injury
Abstract: Diagnosis of myocardial infarction (MI) is based on ST-segment elevation on electrocardiographic evaluation and/or elevated plasma cardiac troponin (cTn) levels. However, troponins lack the sensitivity required to detect the onset of MI at its earliest stages. Therefore, to confirm its viability as an ultra-early biomarker of MI, this study investigates the release kinetics of cardiac myosin binding protein-C (cMyBP-C) in a porcine model of MI and in two human cohorts. Release kinetics of cMyBP-C were determined in a porcine model of MI (n = 6, pigs, either sex) by measuring plasma cMyBP-C level serially from 30 min to 14 days after coronary occlusion, with use of a custom-made immunoassay. cMyBP-C plasma levels were increased from baseline (76 +/- 68 ng/l) at 3 h (767 +/- 211 ng/l) and peaked at 6 h (2,418 +/- 780 ng/l) after coronary ligation. Plasma cTnI, cTnT, and myosin light chain-3 levels were all increased 6 h after ligation. In a cohort of patients (n = 12) with hypertrophic obstructive cardiomyopathy undergoing transcoronary ablation of septal hypertrophy, cMyBP-C was significantly increased from baseline (49 +/- 23 ng/l) in a time-dependent manner, peaking at 4 h (560 +/- 273 ng/l). In a cohort of patients with non-ST segment elevation MI (n = 176) from the SYNERGY trial, cMyBP-C serum levels were significantly higher (7,615 +/- 4,514 ng/l) than those in a control cohort (416 +/- 104 ng/l; n = 153). cMyBP-C is released in the blood rapidly after cardiac damage and therefore has the potential to positively mark the onset of MI.
Journal Title: American Journal of Physiology - Heart Circulatory Physiology
Volume: 306
Issue: 4
ISSN: 0363-6135
Publisher: Unknown  
Journal Place: United States
Date Published: 2014
Start Page: H547
End Page: 56
Language: eng
DOI/URL:

10.1152/ajpheart.00846.2013
Notes: LR: 20150217; GR: 81150/Canadian Institutes of Health Research/Canada; GR: K02HL-114749/HL/NHLBI NIH HHS/United States; GR: R01HL-105826/HL/NHLBI NIH HHS/United States; JID: 100901228; 0 (Biological Markers); 0 (Carrier Proteins); 0 (Troponin I); 0 (Troponin T); 0 (myosin-binding protein C); OID: NLM: PMC3920245; OTO: NOTNLM; 2013/12/13 [aheadofprint]; ppublish