CBX8, a component of the Polycomb PRC1 complex, modulates DOT1L-mediated gene expression through AF9/MLLT3 Journal Article

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Authors: Malik, B.; Hemenway, C. S.
Article Title: CBX8, a component of the Polycomb PRC1 complex, modulates DOT1L-mediated gene expression through AF9/MLLT3
Abstract: AF9 is known to interact with multiple proteins including activators and repressors of transcription. Our data indicate that other AF9 binding proteins compete with the histone methyltransferase DOT1L for AF9 binding thus diminishing its ability to methylate lysine 79 of histone 3. Specifically, we show that AF9 is part of a protein multimer containing members of Polycomb group (PcG) PRC1 complex, CBX8, RING1B, and BMI1. Interaction with CBX8 precludes AF9-DOT1L binding. Knockdown of CBX8 with short-hairpin RNA (shRNA) leads to decreased expression of the AF9 target gene ENaCalpha. In contrast, CBX8 overexpression results in increased ENaCalpha mRNA levels and this effect can be partially overcome by co-overexpression of AF9.
Journal Title: FEBS letters
Volume: 587
Issue: 18
ISSN: 1873-3468; 0014-5793
Publisher: Elsevier Inc  
Journal Place: Netherlands
Date Published: 2013
Start Page: 3038
End Page: 3044
Language: eng
DOI/URL:

10.1016/j.febslet.2013.07.034
Notes: LR: 20141113; CI: Copyright (c) 2013; GR: R01 CA098459/CA/NCI NIH HHS/United States; GR: R01 CA155328/CA/NCI NIH HHS/United States; JID: 0155157; 0 (CBX8 protein, human); 0 (Epithelial Sodium Channels); 0 (MLLT3 protein, human); 0 (Nuclear Proteins); 0 (Polycomb-Group Proteins); 0 (RNA, Messenger); 0 (RNA, Small Interfering); EC 2.1.1.- (DOT1L protein, human); EC 2.1.1.- (Methyltransferases); EC 6.3.2.19 (Polycomb Repressive Complex 1); NIHMS510051; OID: NLM: NIHMS510051; OID: NLM: PMC3800029; OTO: NOTNLM; 2013/04/06 [received]; 2013/07/03 [revised]; 2013/07/05 [accepted]; 2013/07/25 [aheadofprint]; ppublish