Ubiquitin Urine Levels in Burn Patients Journal Article

Authors: Wong, Y. M.; LaPorte, H. M.; Albee, L. J.; Baker, T. A.; Bach, H. H., 4th; Vana, P. G.; Evans, A. E.; Gamelli, R. L.; Majetschak, M
Article Title: Ubiquitin Urine Levels in Burn Patients
Abstract: The objective of this study was to determine whether urine ubiquitin levels are elevated after burns and to assess whether urine ubiquitin could be useful as a noninvasive biomarker for burn patients. Forty burn patients (%TBSA: 20 +/- 22; modified Baux scores: 73 +/- 26) were included (control: 11 volunteers). Urine was collected in 2-hour intervals for 72 hours, followed by 12-hour intervals until discharge from the intensive care unit. Ubiquitin concentrations were analyzed by enzyme linked immunosorbent assay and Western blot. Total protein was determined with a Bradford assay. Patient characteristics and clinical parameters were documented. Urine ubiquitin concentrations, renal ubiquitin excretion, and excretion rates were correlated with patient characteristics and outcomes. Initial urine ubiquitin concentrations were 362 +/- 575 ng/ml in patients and 14 +/- 18 ng/ml in volunteers (P /=20 than with %TBSA 20, in patients who developed sepsis/multiple organ failure than in patients without these complications and in nonsurvivors vs survivors. Ubiquitin urine levels are significantly increased after burns. Renal ubiquitin excretion and/or excretion rates are associated with %TBSA, sepsis/multiple organ failure, and mortality. Although these findings may explain previous correlations between systemic ubiquitin levels and outcomes after burns, the large variability of ubiquitin urine levels suggests that urine ubiquitin will not be useful as a noninvasive disease biomarker.
Journal Title: Journal of burn care research : official publication of the American Burn Association
ISSN: 1559-0488; 1559-047X
Publisher: Unknown  
Date Published: 2015
Language: ENG
Notes: LR: 20150729; GR: T32 GM008750/GM/NIGMS NIH HHS/United States; JID: 101262774; aheadofprint