Noninvasive measurement of intestinal inflammation after burn injury Journal Article

Authors: Sigman, M.; Conrad, P.; Rendon, J. L.; Akhtar, S; Eberhardt, J; Gamelli, R. L.; Choudhry, M. A.
Article Title: Noninvasive measurement of intestinal inflammation after burn injury
Abstract: Intestinal inflammation has been linked with multiorgan failure in patients with burn and other traumatic injuries. We hypothesized that markers of intestinal inflammation are detectible noninvasively. Fecal samples were collected from seven severely burned patients and 15 control patients for the measurement of inflammatory cytokines using a multiplex assay kit. In addition, fecal levels of myeloperoxidase (MPO) and elastase were measured using standard procedures. Compared with a control group, levels of inflammatory cytokines were significantly increased in the burn group. Interleukin (IL)-6 increased to a mean (+/- SEM) of 2.16 +/- 0.61 to 3.81 +/- 0.49 pg/mg (P .05), as did IL-8 (3.32 +/- 0.76 to 20.51 +/- 6.65 pg/mg; P .05), IL-12 (6.23+/-0.98 to 8.11+/-0.95pg/mg; P=0.01), IL-13 (3.86 +/- 0.32 to 11.83 +/- 1.47 pg/mg; P .05), monocyte chemoattractant protein-1 (2.78 +/- 2.61 to 6.5 +/- 3.97 pg/mg; P .05), MPO (13.41 +/- 1.40 to 24.52 +/- 4.31 units/mg protein; P .05), and elastase (2.46 +/- 0.38 to 5.08 +/- 0.72 pg/mL; P .05). Our results suggest that markers of intestinal inflammation are measurable by noninvasive means and are increased after burn injury compared with controls. Of note, increased IL-8 correlated with increased MPO and elastase activity, suggesting a role for neutrophil activation in burn-mediated intestinal inflammation. Thus, these inflammatory cytokine profiles may be valuable biomarkers of intestinal inflammation after burn injury.
Journal Title: Journal of burn care research : official publication of the American Burn Association
Volume: 34
Issue: 6
ISSN: 1559-0488; 1559-047X
Publisher: Unknown  
Journal Place: United States
Date Published: 2013
Start Page: 633
End Page: 638
Language: eng
Notes: GR: F30 AA020167/AA/NIAAA NIH HHS/United States; GR: F30AA020167/AA/NIAAA NIH HHS/United States; GR: R01 AA015731/AA/NIAAA NIH HHS/United States; GR: R01AA015731/AA/NIAAA NIH HHS/United States; JID: 101262774; 0 (Biological Markers); 0 (Cytokines); EC (Peroxidase); EC (Pancreatic Elastase); NIHMS432284; OID: NLM: NIHMS432284 [Available on 11/01/14]; OID: NLM: PMC3690166 [Available on 11/01/14]; PMCR: 2014/11/01 00:00; ppublish