Adipose inflammation and macrophage infiltration after binge ethanol and burn injury Journal Article

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Authors: Qin, Y.; Hamilton, J. L.; Bird, M. D.; Chen, M. M.; Ramirez, L; Zahs, A.; Kovacs, E. J.; Makowski, L.
Article Title: Adipose inflammation and macrophage infiltration after binge ethanol and burn injury
Abstract: BACKGROUND: Ethanol (EtOH) exposure prior to traumatic injury, such as a burn, elevates systemic and local inflammatory responses and increases morbidity and mortality. Adipose is a large tissue mass that is often inflamed during obesity or other stresses, which disturbs metabolic homeostasis. To date, there has been little investigation into the inflammatory response of adipose tissue after combined EtOH exposure and burn injury. METHODS: Two EtOH exposure regimens were utilized to examine the role of inflammation in adipose tissue after EtOH and burn injury. Mice were either given a single or episodic binge exposure to EtOH or saline followed by scald (burn) or sham injury 30 minutes later. Twenty-four hours post injury, serum and adipose tissue were collected for assessment of inflammatory mediators. RESULTS: Single binge EtOH alone induced no inflammation in adipose when compared with sham vehicle-treated mice. However, single binge EtOH followed by burn injury induced significant elevations in mRNA and protein concentrations of pro-inflammatory mediators interleukin-6 (IL-6), KC, and monocyte chemoattractant protein 1 compared with either insult alone or sham vehicle group. Additionally, EtOH exposure and burn injury significantly blunted inducible nitric oxide synthase (iNOS), indicating a complex inflammatory response. Episodic binge EtOH exposure followed by burn injury exacerbated the postburn adipose inflammatory response. The magnitude of the episodic binge-induced inflammatory parameters postburn were 2- to 5-fold greater than the response detected after a single exposure of EtOH, indicating EtOH-induced potentiation of burn-induced inflammatory response. Finally, inflammatory loci and crown-like structures in adipose were significantly increased by episodic binge EtOH and burn injury. CONCLUSIONS: This is the first report of binge and burn-induced crown-like structure formation. Evidence presented herein suggests an important role for alcohol and burn as an additional mediator of adipose inflammation in postburn injury, a common complication in burn patients.
Journal Title: Alcoholism, Clinical and Experimental Research
Volume: 38
Issue: 1
ISSN: 1530-0277; 0145-6008
Publisher: Unknown  
Journal Place: England
Date Published: 2014
Start Page: 204
End Page: 213
Language: eng
DOI/URL:

10.1111/acer.12210
Notes: CI: Copyright (c) 2013; GR: AA017376/AA/NIAAA NIH HHS/United States; GR: ES019472/ES/NIEHS NIH HHS/United States; GR: F30 AA022856/AA/NIAAA NIH HHS/United States; GR: F31 AA019913/AA/NIAAA NIH HHS/United States; GR: F31 AA019913/AA/NIAAA NIH HHS/United States; GR: F32 AA018068/AA/NIAAA NIH HHS/United States; GR: F32 AA018068/AA/NIAAA NIH HHS/United States; GR: P30 DK034987/DK/NIDDK NIH HHS/United States; GR: P30 DK056350/DK/NIDDK NIH HHS/United States; GR: P30DK034987/DK/NIDDK NIH HHS/United States; GR: P30DK056350/DK/NIDDK NIH HHS/United States; GR: R00 AA017376/AA/NIAAA NIH HHS/United States; GR: R01 AA012034/AA/NIAAA NIH HHS/United States; GR: R01 AA012034/AA/NIAAA NIH HHS/United States; GR: T32 AA013527/AA/NIAAA NIH HHS/United States; GR: T32AA013527/AA/NIAAA NIH HHS/United States; GR: U01 ES019472/ES/NIEHS NIH HHS/United States; JID: 7707242; NIHMS496340; OID: NLM: NIHMS496340 [Available on 01/01/15]; OID: NLM: PMC3823749 [Available on 01/01/15]; OTO: NOTNLM; PMCR: 2015/01/01 00:00; 2012/11/02 [received]; 2013/05/06 [accepted]; 2013/08/01 [aheadofprint]; ppublish