Insulin resistance induces medial artery calcification in fructose-fed rats Journal Article

Authors: Zhou, Y. B.; Zhang, J; Cai, Y; Teng, X.; Duan, X. H.; Song, J. Q.; Du, J.; Tang, C. S.; Qi, Y. F.
Article Title: Insulin resistance induces medial artery calcification in fructose-fed rats
Abstract: Osteogenic differentiation of vascular smooth muscle cells (VSMCs) results in medial artery calcification, which is common in diabetes, but the pathogenesis is poorly understood. We aimed to explore the pathophysiological roles of insulin resistance (IR) on medial artery calcification in rats with 10% fructose in drinking water. After 12 weeks of fructose feeding, rats showed severe IR, with increased levels of fasting blood glucose, serum insulin and oral glucose tolerance test (OGTT). Fructose-fed rats showed aortic calcification, increased aortic calcium deposition and irregular elastic fibers in the medial layer of the vessel wall. Moreover, plasma phosphorus concentration, calcium x phosphorus product and alkaline phosphatase (ALP) activity, and aortic calcium content and ALP activity were significantly increased. Fructose feeding increased mRNA levels of osteopontin, type III sodium-dependent phosphate co-transporter, bone morphogenetic protein-2 and the key transcription factor core binding factor alpha 1 in aortic tissue and downregulated mRNA levels of osteoprotegerin and matrix gamma-carboxyglutamic acid protein. Fructose feeding decreased protein levels of smooth-muscle lineage markers and induced severe lipid peroxidation injury. IR induced by high fructose feeding could evoke osteogenic transdifferentiation of VSMCs and promote vascular calcification.
Journal Title: Experimental biology and medicine (Maywood, N.J.)
Volume: 237
Issue: 1
ISSN: 1535-3699; 1535-3699
Publisher: Unknown  
Journal Place: England
Date Published: 2012
Start Page: 50
End Page: 57
Language: eng
Notes: JID: 100973463; 0 (Blood Glucose); 0 (Bone Morphogenetic Proteins); 0 (Calcium-Binding Proteins); 0 (Core Binding Factor Alpha 1 Subunit); 0 (Dietary Carbohydrates); 0 (Extracellular Matrix Proteins); 0 (Insulin); 0 (Osteoprotegerin); 0 (RNA, Messenger); 0 (Sodium-Phosphate Cotransporter Proteins, Type III); 0 (matrix Gla protein); 106441-73-0 (Osteopontin); 30237-26-4 (Fructose); 7440-70-2 (Calcium); 7723-14-0 (Phosphorus); EC (Alkaline Phosphatase); ppublish