Generation of rabbit monoclonal antibodies Journal Article

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Authors: Yam, P. C.; Knight, K. L.
Article Title: Generation of rabbit monoclonal antibodies
Abstract: Rabbit hybridomas are gaining wide acceptance as serologic reagents to identify a variety of antigens, including proteins, small peptides, phosphorylated proteins, and polysaccharides. Rabbits make high-affinity IgG antibodies, all of which bind with high affinity to Protein A from Staphylococcus aureus and Protein G from Group G Streptococcus. Consequently, rabbit monoclonal antibodies of desired specificity can be rapidly detected using Protein A/G as secondary reagents. Here we describe the method for generating rabbit monoclonal antibodies using the rabbit hybridoma fusion partner 240E-1. The method begins with the immunization of rabbits and ends with the cloning the antigen-specific hybridomas.
Journal Title: Methods in Molecular Biology
Volume: 1131
ISSN: 1940-6029
Publisher: Unknown  
Journal Place: United States
Date Published: 2014
Start Page: 71
End Page: 79
Language: eng
DOI/URL:

10.1007/978-1-62703-992-5_5

10.1007/978-1-62703-992-5_5
Notes: JID: 9214969; ppublish