Interleukin-22 modulates gut epithelial and immune barrier functions following acute alcohol exposure and burn injury Journal Article

Authors: Rendon, J. L.; Li, X; Akhtar, S; Choudhry, M. A.
Article Title: Interleukin-22 modulates gut epithelial and immune barrier functions following acute alcohol exposure and burn injury
Abstract: Interleukin-22 (IL-22) maintains gut epithelial integrity and expression of antimicrobial peptides Reg3beta and Reg3gamma. Our laboratory has shown that acute alcohol/ethanol (EtOH) exposure before burn injury results in increased gut permeability, intestinal T-cell suppression, and enhanced bacterial translocation. Herein, we determined the effect of combined EtOH intoxication and burn injury on intestinal levels of IL-22 as well as Reg3beta and Reg3gamma expression. We further examined whether in vivo restitution of IL-22 restores gut permeability, Reg3beta and Reg3gamma levels, and bacterial load (e.g., gut bacterial growth) within the intestine after EtOH and burn injury. Male mice, approximately 25g, were gavaged with EtOH (2.9 mg/kg) before receiving a approximately 12.5% total-body-surface-area, full-thickness burn. Mice were immediately treated with saline control or IL-22 (1 mg/kg) by i.p. injection. One day after injury, there was a significant decrease in intestinal IL-22, Reg3beta, and Reg3gamma expression along with an increase in intestinal permeability and gut bacterial load after EtOH combined with burn injury, as compared with sham injury. Treatment with IL-22 normalized Reg3beta and Reg3gamma expression and attenuated the increase in intestinal permeability after EtOH and burn injury. Qualitatively, IL-22 treatment reduced the bacterial load in nearly half of mice receiving EtOH combined with burn injury. Our data indicate that IL-22 maintains gut epithelial and immune barrier integrity after EtOH and burn injury; thus, the IL-22/antimicrobial peptide pathway may provide a therapeutic target for the treatment of patients who sustain burn injury under the influence of EtOH.
Journal Title: Shock
Volume: 39
Issue: 1
ISSN: 1540-0514; 1073-2322
Publisher: Unknown  
Journal Place: United States
Date Published: 2013
Start Page: 11
End Page: 18
Language: eng
Notes: ID: 13147; GR: F30 AA020167/AA/NIAAA NIH HHS/United States; GR: F30AA020167/AA/NIAAA NIH HHS/United States; GR: R01 AA015731/AA/NIAAA NIH HHS/United States; GR: R01AA015731/AA/NIAAA NIH HHS/United States; GR: R01AA015731-04S1/AA/NIAAA NIH HHS/United States; GR: T32 AA013527/AA/NIAAA NIH HHS/United States; GR: T32AA013527/AA/NIAAA NIH HHS/United States; JID: 9421564; NIHMS415370; OID: NLM: NIHMS415370 [Available on 01/01/14]; OID: NLM: PMC3526677 [Available on 01/01/14]; PMCR: 2014/01/01 00:00; ppublish