Ethanol suppresses phagosomal adhesion maturation, Rac activation, and subsequent actin polymerization during FcgammaR-mediated phagocytosis. Journal Article

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Authors: Karavitis, J.; Murdoch, E. L.; Deburghgraeve, C.; Ramirez, L; Kovacs, E. J.
Article Title: Ethanol suppresses phagosomal adhesion maturation, Rac activation, and subsequent actin polymerization during FcgammaR-mediated phagocytosis.
Abstract: Clinical and laboratory investigations have provided evidence that ethanol suppresses normal lung immunity. Our initial studies revealed that acute ethanol exposure results in transient suppression of phagocytosis of Pseudomonas aeruginosa by macrophages as early as 3 h after initial exposure. Focusing on mechanisms by which ethanol decreases macrophage Fcgamma-receptor (FcgammaR) phagocytosis we targeted the study on the focal adhesion and cytoskeletal elements that are necessary for phagosome progression. Ethanol inhibited macrophage phagocytosis of IgG-coated bead recruitment of actin to the site of the phagosome, dampened the phosphorylation of vinculin, but had no effect on paxillin phosphorylation suggesting a loss in "phagosomal adhesion" maturation. Moreover, our observations revealed that FcgammaR-phagocytosis induced Rac activation, which was increased by only 50% in ethanol exposed cells, compared to 175% in the absence of ethanol. This work is the first to show evidence of the cellular mechanisms involved in the ethanol-induced suppression of FcgammaR-mediated phagocytosis. Copyright Copyright 2012 Elsevier Inc. All rights reserved.
Journal Title: Cellular immunology
Volume: 274
Issue: 1-2
ISSN: 1090-2163; 0008-8749
Publisher: Elsevier Inc  
Journal Place: United States
Date Published: 2012
Start Page: 61
End Page: 71
Language: English
DOI/URL:

http://ovidsp.ovid.com/ovidweb.cgi?T=JS=Y=N=fulltext=medl=22381996
Notes: ID: 12359; Record Owner: From MEDLINE, a database of the U.S. National Library of Medicine.; Status: MEDLINE; Publishing Model: Journal available in: Print-Electronic Citation processed from: Internet; NLM Journal Code: cq9, 1246405; Other ID: Source: NLM. NIHMS357073 [Available on 02/13/13] Source: NLM. PMC3334404 [Available on 02/13/13]; CAS Registry/EC Number/Name of Substance: 0 (Actins). 0 (Paxillin). 0 (Receptors, IgG). 125361-02-6 (Vinculin). 64-17-5 (Ethanol). EC 3-6-5-2 (rac GTP-Binding Proteins). EC 3-6-5-2 (rho GTP-Binding Proteins).; Grant Number: F31 AA017027 (United States NIAAA NIH HHS), F31 AA017032 (United States NIAAA NIH HHS), P30 AA019373-02 (United States NIAAA NIH HHS), R01 AA012034 (United States NIAAA NIH HHS), R01 AA012034 (United States NIAAA NIH HHS), R01 AA012034-10 (United States NIAAA NIH HHS), R01 AA012034-11 (United States NIAAA NIH HHS), R01 AA012034-11S1 (United States NIAAA NIH HHS), R01 AA012034-12 (United States NIAAA NIH HHS), T32 AA013527 (United States NIAAA NIH HHS), T32 AA013527-08 (United States NIAAA NIH HHS), T32 AA013527-09 (United States NIAAA NIH HHS); Electronic Date of Publication: 20120213; Entry Date: 20120614