Determination of Burn Patient Outcome by Large-Scale Quantitative Discovery Proteomics Journal Article

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Authors: Finnerty, C. C.; Jeschke, M. G.; Qian, W. J.; Kaushal, A.; Xiao, W.; Liu, T; Gritsenko, M. A.; Moore, R. J.; Camp, D. G., 2nd; Moldawer, L. L.; Elson, C.; Schoenfeld, D.; Gamelli, R.; Gibran, N.; Klein, M; Arnoldo, B.; Remick, D.; Smith, R. D.; Davis, R.; Tompkins, R. G.; Herndon, D. N.
Article Title: Determination of Burn Patient Outcome by Large-Scale Quantitative Discovery Proteomics
Abstract: OBJECTIVES:: Emerging proteomics techniques can be used to establish proteomic outcome signatures and to identify candidate biomarkers for survival following traumatic injury. We applied high-resolution liquid chromatography-mass spectrometry and multiplex cytokine analysis to profile the plasma proteome of survivors and nonsurvivors of massive burn injury to determine the proteomic survival signature following a major burn injury. DESIGN:: Proteomic discovery study. SETTING:: Five burn hospitals across the United States. PATIENTS:: Thirty-two burn patients (16 nonsurvivors and 16 survivors), 19-89 years old, were admitted within 96 hours of injury to the participating hospitals with burns covering more than 20% of the total body surface area and required at least one surgical intervention. INTERVENTIONS:: None. MEASUREMENTS AND MAIN RESULTS:: We found differences in circulating levels of 43 proteins involved in the acute-phase response, hepatic signaling, the complement cascade, inflammation, and insulin resistance. Thirty-two of the proteins identified were not previously known to play a role in the response to burn. Interleukin-4, interleukin-8, granulocyte macrophage colony-stimulating factor, monocyte chemotactic protein-1, and beta2-microglobulin correlated well with survival and may serve as clinical biomarkers. CONCLUSIONS:: These results demonstrate the utility of these techniques for establishing proteomic survival signatures and for use as a discovery tool to identify candidate biomarkers for survival. This is the first clinical application of a high-throughput, large-scale liquid chromatography-mass spectrometry-based quantitative plasma proteomic approach for biomarker discovery for the prediction of patient outcome following burn, trauma, or critical illness.
Journal Title: Critical Care Medicine
Volume: 41
Issue: 6
ISSN: 1530-0293; 0090-3493
Publisher: Unknown  
Date Published: 2013
Start Page: 1421
End Page: 1434
Language: ENG
DOI/URL:

10.1097/CCM.0b013e31827c072e
Notes: ID: 12965; GR: P41 GM103493/GM/NIGMS NIH HHS/United States; GR: P41 RR018522/RR/NCRR NIH HHS/United States; GR: P50 GM060338/GM/NIGMS NIH HHS/United States; GR: R01 GM056687/GM/NIGMS NIH HHS/United States; GR: T32 GM008256/GM/NIGMS NIH HHS/United States; GR: U54 GM062119/GM/NIGMS NIH HHS/United States; JID: 0355501; ppublish