Effects of activated omental cells on rat limbal corneal alkali injury Journal Article


Authors: Bu, P; Vin, A. P.; Sethupathi, P.; Ambrecht, L. A.; Zhai, Y.; Nikolic, N.; Qiao, L; Bouchard, C. S.
Article Title: Effects of activated omental cells on rat limbal corneal alkali injury
Abstract: Omental cells (OCs) are shown to help wound healing. The purpose of this study is to investigate if OCs improve cornea repair after alkali injury by subconjunctival injection of activated OCs in rats. Forty eight hours after limbal corneal alkali injury, fresh isolated OCs were injected subconjunctivally into the recipient rat's eye. Prior to the injury and at 0, 4 and 8 days after injury, the eyes were examined using slit lamp biomicroscopy. Corneal opacification and corneal neovascularization were graded in a masked fashion. The inflammatory response to the injury was evaluated by counting neutrophil cell numbers in the cornea under microscope. There was no significant difference in corneal opacification between the control and OCs treatment groups; however, the corneal neovascularization was significantly less in the eyes treated with OCs as compared to the controls. Also OCs treatment markedly decreased neutrophil infiltration after corneal-limbal alkali injury. Our results suggest that OCs may have a beneficial role in corneal healing after limbal corneal alkali injury by suppressing inflammatory cell infiltrates and corneal neovascularization.
Journal Title: Experimental eye research
ISSN: 1096-0007; 0014-4835
Publisher: Elsevier Inc  
Date Published: 2014
Start Page: 143
End Page: 146
Language: ENG
DOI/URL:
Notes: CI: Copyright (c) 2014; JID: 0370707; OTO: NOTNLM; 2013/08/01 [received]; 2014/01/27 [revised]; 2014/02/15 [accepted]; aheadofprint