Enhancement of antigen-specific CD4+ and CD8+ T cell responses using a self-assembled biologic nanolipoprotein particle vaccine Journal Article


Authors: Weilhammer, D.; Dunkle, A. D.; Blanchette, C. D.; Fischer, N. O.; Corzett, M.; Lehmann, D.; Boone, T.; Hoeprich, P.; Driks, A.; Rasley, A.
Article Title: Enhancement of antigen-specific CD4+ and CD8+ T cell responses using a self-assembled biologic nanolipoprotein particle vaccine
Abstract: To address the need for vaccine platforms that induce robust cell-mediated immunity, we investigated the potential of utilizing self-assembling biologic nanolipoprotein particles (NLPs) as an antigen and adjuvant delivery system to induce antigen-specific murine T cell responses. We utilized OT-I and OT-II TCR-transgenic mice to investigate the effects of NLP-mediated delivery of the model antigen ovalbumin (OVA) on T cell activation. Delivery of OVA with the TLR4 agonist monophosphoryl lipid A (MPLA) in the context of NLPs significantly enhanced the activation of both CD4+ and CD8+ T cells in vitro compared to co-administration of free OVA and MPLA. Upon intranasal immunization of mice harboring TCR-transgenic cells, NLPs enhanced the adjuvant effects of MPLA and the in vivo delivery of OVA, leading to significantly increased expansion of CD4+ and CD8+ T cells in lung-draining lymph nodes. Therefore, NLPs are a promising vaccine platform for inducing T cell responses following intranasal administration.
Keywords: Microbiology and Immunology; Vaccine; Intranasal; Nanodisc; Nanolipoprotein; Nanoparticle; T cell activation
Journal Title: Vaccine
Volume: 35
Issue: 11
ISSN: 1873-2518; 0264-410X
Publisher: Elsevier Inc  
Journal Place: Netherlands
Date Published: 2017
Start Page: 1475
End Page: 1481
Language: eng
DOI/URL:
Notes: LR: 20170228; CI: Copyright (c) 2017; JID: 8406899; OTO: NOTNLM; 2016/09/21 [received]; 2017/01/23 [revised]; 2017/02/04 [accepted]; ppublish