Acute inhibitory effect of alpha-mangostin on sarcoplasmic reticulum calcium-ATPase and myocardial relaxation Journal Article

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Authors: Phungphong, S.; Kijtawornrat, A.; de Tombe, P. P.; Wattanapermpool, J.; Bupha-Intr, T.; Suksamrarn, S.
Article Title: Acute inhibitory effect of alpha-mangostin on sarcoplasmic reticulum calcium-ATPase and myocardial relaxation
Abstract: The benefits of alpha-mangostin for various tissues have been reported, but its effect on the heart has not been clarified. This study aimed to evaluate the effects of alpha-mangostin on cardiac function. Using a cardiac sarcoplasmic reticulum (SR) membrane preparation, alpha-mangostin inhibited SR Ca2+ -ATPase activity in a dose-dependent manner (IC50 of 6.47 +/- 0.7 muM). Its suppressive effect was specific to SR Ca2+ -ATPase but not to myofibrillar Ca2+ -ATPase. Using isolated cardiomyocytes, 50 muM of alpha-mangostin significantly increased the duration of cell relengthening and increased the duration of Ca2+ transient decay, suggesting altered myocyte relaxation. The relaxation effect of alpha-mangostin was also supported in vivo after intravenous infusion. A significant suppression of both peak pressure and rate of ventricular relaxation (-dP/dt) relative to DMSO infusion was observed. The results from the present study demonstrated that alpha-mangostin exerts specific inhibitory action on SR Ca2+ -ATPase activity, leading to myocardial relaxation dysfunction.
Journal Title: Journal of Biochemical and Molecular Toxicology
ISSN: 1099-0461; 1095-6670
Publisher: Wiley Periodicals, Inc  
Journal Place: United States
Date Published: 2017
Language: eng
DOI/URL:

10.1002/jbt.21942
Notes: LR: 20170607; CI: (c) 2017; JID: 9717231; OTO: NOTNLM; 2017/03/21 [received]; 2017/05/17 [revised]; 2017/05/20 [accepted]; aheadofprint