In vitro evidence that RNA Polymerase acetylation and acetyl phosphate-dependent CpxR phosphorylation affect cpxP transcription regulation Journal Article

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Authors: Lima, B. P.; Lennon, C. W.; Ross, W.; Gourse, R. L.; Wolfe, A. J.
Article Title: In vitro evidence that RNA Polymerase acetylation and acetyl phosphate-dependent CpxR phosphorylation affect cpxP transcription regulation
Abstract: The central metabolite acetyl phosphate (acP) has long been proposed to influence transcription regulation by directly transferring its phosphoryl group to a number of response regulators in many bacterial species. Here, we provide in vitro evidence for this proposition and demonstrate, using an in vitro transcription system, that acP-dependent phosphorylation of aspartate 51 of CpxR induces transcription of one of its regulon members in E. coli, cpxP. We also used this in vitro transcription system to extend our previously reported in vivo data that hypothesized that acetylation of RNA polymerase (RNAP) influences acP-dependent cpxP transcription, using glutamine as a genetic mimic for acetylated arginine 291 of the carboxy-terminal domain of RNAP alpha subunit. The data we present here lend strong support to the hypothesis that acP has a direct effect on transcription regulation in E. coli via phosphorylation of CpxR, and that RNAP acetylation can modulate this response.
Journal Title: FEMS microbiology letters
Volume: 363
Issue: 5
ISSN: 1574-6968; 0378-1097
Publisher: Unknown  
Journal Place: England
Date Published: 2016
Start Page: 10.1093/femsle/fnw011. Epub 2016 Jan 19
Language: eng
DOI/URL:

10.1093/femsle/fnw011
Notes: LR: 20160506; CI: (c) FEMS 2016; GR: R37 GM037048/GM/NIGMS NIH HHS/United States; JID: 7705721; OTO: NOTNLM; 2016/01/14 [accepted]; 2016/01/19 [aheadofprint]; ppublish