Age-dependent Effects of 17beta-estradiol on the dynamics of estrogen receptor beta (ERbeta) protein-protein interactions in the ventral hippocampus Journal Article

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Authors: Mott, N. N.; Pinceti, E.; Rao, Y. S.; Przybycien-Szymanska, M. M.; Prins, S. A.; Shults, C. L.; Yang, X.; Glucksman, M. J.; Roberts, J. L.; Pak, T. R.
Article Title: Age-dependent Effects of 17beta-estradiol on the dynamics of estrogen receptor beta (ERbeta) protein-protein interactions in the ventral hippocampus
Abstract: Recent clinical evidence suggests that the neuroprotective and beneficial effects of hormone therapy may be limited by factors related to age and reproductive status. The patient's age and length of time without circulating ovarian hormones are likely to be key factors in the specific neurological outcomes of hormone therapy. However, the mechanisms underlying age-related changes in hormone efficacy have not been determined. We hypothesized that there are intrinsic changes in estrogen receptor beta (ERbeta) function that determine its ability to mediate the actions of 17beta-estradiol (E2) in brain regions such as the ventral hippocampus. In this study, we identified and quantified a subset of ERbeta protein interactions in the ventral hippocampus that were significantly altered by E2 replacement in young and aged animals, using two-dimensional differential gel electrophoresis coupled with liquid chromatography-electrospray ionization-tandem mass spectrometry. This study demonstrates quantitative changes in ERbeta protein-protein interactions with E2 replacement that are dependent upon age in the ventral hippocampus and how these changes could alter processes such as transcriptional regulation. Thus, our data provide evidence that changes in ERbeta protein interactions are a potential mechanism for age-related changes in E2 responsiveness in the brain after menopause.
Journal Title: Molecular cellular proteomics : MCP
Volume: 13
Issue: 3
ISSN: 1535-9484; 1535-9476
Publisher: Unknown  
Journal Place: United States
Date Published: 2014
Start Page: 760
End Page: 779
Language: eng
DOI/URL:

10.1074/mcp.M113.031559
Notes: LR: 20150311; GR: NIH F31 AG039931/AG/NIA NIH HHS/United States; GR: NIH OD010662/OD/NIH HHS/United States; GR: NIH R01AG033605/AG/NIA NIH HHS/United States; JID: 101125647; 0 (Annexin A5); 0 (Cell Cycle Proteins); 0 (Estrogen Receptor beta); 0 (Gelsolin); 0 (RNA, Small Interfering); 4TI98Z838E (Estradiol); EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases); EC 3.6.1.- (Adenosine Triphosphatases); EC 3.6.1.- (CDC48 protein); OID: NLM: PMC3945907; 2014/01/05 [aheadofprint]; ppublish