WEE1 is a validated target of the microRNA miR-17-92 cluster in leukemia Journal Article

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Authors: Brockway, S.; Zeleznik-Le, N. J.
Article Title: WEE1 is a validated target of the microRNA miR-17-92 cluster in leukemia
Abstract: MicroRNAs are short single-stranded RNAs that regulate target gene expression by binding to complementary sites in the 3' untranslated region (UTR) of their mRNA targets. The polycistronic miR-17-92 cluster, which encodes miR-17, miR-18a, miR-19a, miR-20a, miR-19b, and miR-92a, was previously shown to be overexpressed in multiple types of cancer. In this study, target gene prediction algorithms were used to predict potential targets of the miR-17-92 cluster. WEE1, a kinase that inhibits cell cycle progression, was identified as a possible target of five of the six miRNAs in the cluster. Luciferase reporter assays were used to determine that miR-17, miR-20a, and miR-18a specifically target nucleotides 465-487 of the 3' UTR of WEE1, whereas miR-19a and miR-19b exert control on WEE1 by targeting nucleotides 1069-1091. A negative correlation was determined between endogenous miR-17 or miR-19a expression and endogenous WEE1 protein expression in the same panel of cell lines. We conclude that WEE1 is a valid target of the miR-17-92 cluster in leukemia.
Journal Title: Cancer genetics
Volume: 208
Issue: 5
ISSN: 2210-7762
Publisher: Elsevier Inc  
Date Published: 2015
Start Page: 279
End Page: 287
Language: ENG
DOI/URL:

S2210-7762(15)00002-2
Notes: LR: 20150323; CI: Copyright (c) 2015; GR: R01 HL087188/HL/NHLBI NIH HHS/United States; JID: 101539150; OTO: NOTNLM; 2014/09/09 [received]; 2015/01/04 [revised]; 2015/01/11 [accepted]; aheadofprint