Regional variation in expression of pro-inflammatory mediators in the intestine following a combined insult of alcohol and burn injury Journal Article

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Authors: Morris, N. L.; Li, X; Earley, Z. M.; Choudhry, M. A.
Article Title: Regional variation in expression of pro-inflammatory mediators in the intestine following a combined insult of alcohol and burn injury
Abstract: The intestine is segmented into functionally discrete compartments (duodenum, jejunum, ileum, and colon). The present study examined whether alcohol combined with burn injury differently influences cytokine levels in different parts of the intestine. Male mice were gavaged with alcohol ( approximately 2.9 g/kg) 4 h prior to receiving a approximately 12.5% total body surface area full thickness burn. Mice were sacrificed 1, 3, and 7 days after injury. The intestine segments (duodenum, jejunum, ileum, and colon) were harvested, homogenized, and analyzed for inflammatory mediators (IL-6, IL-18, and KC) using their respective ELISAs. KC levels were significantly increased in the jejunum, ileum, and colon following alcohol and burn injury as compared to shams. The increase in KC was approximately 28-fold higher in the colon as compared to the levels observed in duodenum following alcohol and burn injury. Both IL-6 and IL-18 levels were significantly elevated in both the ileum and colon following the combined insult. There was a approximately 7-fold increase in IL-6 levels in the colon as compared with the duodenum after the combined insult. Levels of IL-18 were increased by approximately 1.5-fold in the colon as compared to the ileum following alcohol and burn injury. The data suggest that pro-inflammatory mediators are differentially expressed in the intestine following alcohol and burn injury.
Journal Title: Alcohol (Fayetteville, N.Y.)
Volume: 49
Issue: 507
ISSN: 1873-6823; 0741-8329
Publisher: Unknown  
Date Published: 2015
Start Page: 511
Language: ENG
DOI/URL:

S0741-8329(14)20216-3
Notes: LR: 20150505; CI: Copyright (c) 2015; GR: R01 AA015731/AA/NIAAA NIH HHS/United States; GR: T32 AA013527/AA/NIAAA NIH HHS/United States; JID: 8502311; NIHMS675638; OTO: NOTNLM; PMCR: 2016/09/26 00:00; 2014/10/31 [received]; 2015/01/29 [revised]; 2015/02/10 [accepted]; aheadofprint