Elimination of the actin-binding domain in kelch-like 1 protein induces T-type calcium channel modulation only in the presence of action potential waveforms Journal Article

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Authors: Aromolaran, K. A.; Benzow, K. A.; Cribbs, L. L.; Koob, M. D.; Piedras-Renteria, E. S.
Article Title: Elimination of the actin-binding domain in kelch-like 1 protein induces T-type calcium channel modulation only in the presence of action potential waveforms
Abstract: The Kelch-like 1 protein (KLHL1) is a neuronal actin-binding protein that modulates calcium channel function. It increases the current density of Ca(v)3.2 (alpha(1H)) calcium channels via direct interaction with alpha(1H) and actin-F, resulting in biophysical changes in Ca(v)3.2 currents and an increase in recycling endosomal activity with subsequent increased alpha(1H) channel number at the plasma membrane. Interestingly, removal of the actin-binding Kelch motif (DeltaKelch) prevents the increase in Ca(v)3.2 current density seen with wild-type KLHL1 when tested with normal square pulse protocols but does not preclude the effect when tested using action potential waveforms (AP). Here, we dissected the kinetic properties of the AP waveform that confer the mutant Kelch the ability to interact with Ca(v)3.2 and induce an increase in calcium influx. We modified the action potential waveform by altering the slopes of repolarization and/or recovery from hyperpolarization or by changing the duration of the depolarization plateau or the hyperpolarization phase and tested the modulation of Ca(v)3.2 by the mutant DeltaKelch. Our results show that the recovery phase from hyperpolarization phase determines the conformational changes that allow the alpha(1H) subunit to properly interact with mutant KLHL1 lacking its actin-binding Kelch domains, leading to increased Ca influx.
Journal Title: Journal of signal transduction
Volume: 2012
ISSN: 2090-1747; 2090-1747
Publisher: Unknown  
Journal Place: United States
Date Published: 2012
Start Page: 505346
Language: eng
DOI/URL:

10.1155/2012/505346
Notes: LR: 20130402; JID: 101536934; OID: NLM: PMC3401526; 2012/04/06 [received]; 2012/05/30 [accepted]; 2012/07/11 [epublish]; ppublish