Production of Anti-platelet Factor 4/Heparin Complex Antibodies After Cardiovascular Surgery Journal Article

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Authors: Matsuo, T.; Motohashi, S; Wanaka, K.; Walenga, J. M.
Article Title: Production of Anti-platelet Factor 4/Heparin Complex Antibodies After Cardiovascular Surgery
Abstract: To study the production of anti-platelet factor 4 (anti-PF4)/heparin complex antibodies of Ig (immunoglobulin) G/IgA/IgM using enzyme-linked immunosorbent assay (ELISA; heparin-induced thrombocytopenia [HIT] antibodies) in 79 patients undergoing cardiovascular surgery, we employed Deltaoptical density (OD) as a marker of HIT-antibody production. The DeltaODs were calculated from the differences in the ODs using ELISA. Patient were classified into 3 DeltaOD ranges: DeltaOD >/= 1.0, DeltaOD >/= 0.4 to d-dimer level, postoperative brain magnetic resonance imaging (MRI), use of cardiopulmonary bypass and postoperative thrombocytosis were not considered for the 3 DeltaOD classifications. None of the 6 patients with DeltaOD >/=1.0 and a positive functional assay was diagnosed with HIT due to the absence of HIT-derived thrombocytopenia. In conclusion, HIT-antibody production increased until day 7 after heparin cessation and reached a trace level on day 14. It was demonstrated that HIT-antibody production is in remission unless there is any evidence of a further increase during the second week postsurgery.
Journal Title: Clinical Applied Thrombosis/Hemostasis
Volume: 21
Issue: 2
ISSN: 1938-2723
Publisher: Unknown  
Date Published: 2015
Start Page: 177
End Page: 180
Language: ENG
DOI/URL:

10.1177/1076029613507334
Notes: CI: (c) The Author(s) 2013; JID: 9508125; OTO: NOTNLM; 2013/11/06 [aheadofprint]; ppublish