Alteration in intestine tight junction protein phosphorylation and apoptosis is associated with increase in IL-18 levels following alcohol intoxication and burn injury. Journal Article

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Authors: Li, X; Akhtar, S; Choudhry, M. A.
Article Title: Alteration in intestine tight junction protein phosphorylation and apoptosis is associated with increase in IL-18 levels following alcohol intoxication and burn injury.
Abstract: Intestinal mucosal barrier is the first line of defense against bacteria and their products originating from the intestinal lumen. We have shown a role for IL-18 in impaired gut barrier function following acute alcohol (EtOH) intoxication combined with burn injury. To further delineate the mechanism, this study examined whether IL-18 alters intestine tight junction proteins or induces mucosal apoptosis under these conditions. To accomplish this, rats were gavaged with EtOH (3.2g/kg) prior to ~12.5% total body surface area burn or sham injury. One day after injury, EtOH combined with burn injury resulted in a significant decrease in total occludin protein and its phosphorylation in small intestine compared to either EtOH or burn injury alone. There was no change in claudin-1 protein content but its phosphorylation on tyrosine was decreased following EtOH and burn injury. This was accompanied with an increase in mucosal apoptosis (p0.05). The treatment of rats with anti-IL-18 antibody at the time of burn injury prevented intestine apoptosis and normalized tight junction proteins following EtOH and burn injury. Altogether, these findings suggest that IL-18 modulates tight junction proteins and cause apoptosis leading to impaired intestinal mucosal integrity following EtOH intoxication combined with burn injury. Copyright Copyright 2011 Elsevier B.V. All rights reserved.
Journal Title: Biochimica et biophysica acta
Volume: 1822
Issue: 2
ISSN: 0006-3002; 0006-3002
Publisher: Elsevier Inc  
Journal Place: Netherlands
Date Published: 2012
Start Page: 196
End Page: 203
Language: English
DOI/URL:

http://ovidsp.ovid.com/ovidweb.cgi?T=JS=Y=N=fulltext=medl=22001439

http://hn9yf5lh6v.search.serialssolutions.com/?url_ver=Z39.88-2004=info:ofi/fmt:kev:mtx:journal=info:sid/Ovid:medl=article=info:doi/=info:pmid/22001439=0006-3002=1822=2=196=196-203=2012=Biochimica+et+Biophysica+Acta=Alteration+in+intestine+tight+junction+protein+phosphorylation+and+apoptosis+is+associated+with+increase+in+IL-18+levels+following+alcohol+intoxication+and+burn+injury.=Li
Notes: ID: 12452; Record Owner: From MEDLINE, a database of the U.S. National Library of Medicine.; Status: MEDLINE; Publishing Model: Journal available in: Print-Electronic Citation processed from: Print; NLM Journal Code: a0w, 0217513; Other ID: Source: NLM. NIHMS345305 [Available on 02/01/13] Source: NLM. PMC3261659 [Available on 02/01/13]; CAS Registry/EC Number/Name of Substance: 0 (Antibodies). 0 (Interleukin-18). 0 (Membrane Proteins). 0 (claudin 1). 0 (occludin). 55520-40-6 (Tyrosine). EC 3-4-22 (Caspase 3).; Grant Number: R01 AA015731-01A2 (United States NIAAA NIH HHS), R01AA015731 (United States NIAAA NIH HHS), R21 AA015979-01A1 (United States NIAAA NIH HHS), R21AA015979 (United States NIAAA NIH HHS); Electronic Date of Publication: 20111007; Entry Date: 20120710